Abstract
The effects of increased glucose concentration in blood on the glucose effect proposed in our previous papers were studied with sulfisoxazole, metoclopramide, and sulfanilamide using the in situ recirculating perfusion method with perfusion solution having three different tonicities which were adjusted to hypertonic, isotonic, and hypotonic with sodium chloride or glucose. To achieve the purpose, alloxan-induced diabetic rats were introduced in the present study. No effect of the increase of glucose concentration in blood in the diabetics on the glucose effect was found, since the regression lines representing the relation between the transmucosal fluid movement and the drug absorption with the controls were overlapped to each other with those of the diabetics in both of the media. Simultaneously, the effects of diabetes on the transmucosal fluid movement and the absorption of the drugs were examined. The fluid movement and the absorption of the drugs in the diabetics were always significantly greater than in the controls in all of the experimental conditions. It is reasonable to understand that the increased drug absorptions might be based on only the increment in the transmucosal fluid inflow.
