Abstract
A fluorometric method is presented for the assay of dopamine-β-hydroxylase activity in human serum. This is based on the enzymatic conversion of β-phenylethylamine under the optimum conditions to phenylethanolamine, which is then oxidized to benzaldehyde and determined fluorometrically by means of the previously established method for selective determination of aromatic aldehydes with 1, 2-diaminonaphthalene. The method is readily performed and is suitable to assay a large number of samples at the same time with 10μl of sample.
