Abstract
The conidia of Cochliobolus miyabeanus were extracted repeatedly with CHCl3-MeOH mixture (2 : 1) and the contents of their lipids, fatty acid composition of these lipids, and the changes of the fatty acid content with advance of germinating processes in conidia were examined. The results of these examinations were as follows. (1) Total lipid compositions : The extractable lipid and bound lipid contents were 6.3-7.5% and 0.3-1.2% of conidial weight, respectively, and the extractable lipid was composed of 1.54-2.85% triglyceride, 0.12-0.36% diglyceride, 0.22-0.66% monoglyceride, 0.72-1.17% free fatty acid, 0.09-0.10% steroid ester, 1.08-2.49% steroid, and 0.99-1.24% phospholipid of conidial weight. (2) Fatty acid composition : The pattern of fatty acid composition in each fraction of the extractable lipids was almost identical and C16 : 0, C18 : 0, C18 : 1, C18 : 2, and C18 : 3 fatty acids were found in each fraction as dominant constituent. These 5 kinds of fatty acids constituted 85% of total conidial lipids. (3) Changes in fatty acid composition during the germination processes : Although a slight change in fatty acid compositions was found in each lipid fraction during all stages of conidial germination, the content of C18 : 2 in the fraction of phospholipid was inclined to increase with the advancement of germinating process.
