Abstract
Colorimetric and fluorometric methods for the determination of Bunte salts have been developed. Bunte salts are reduced with dithiothreitol (DTT) to monothiols and bisulfite. The latter is determined colorimetrically with a basic fuchsine reagent in the presence of mercuric chloride or fluorometrically with N-(p-dimethylaminophenyl)-1, 4-naphthoquinoneimine in the presence of a mixture of p-chloromercuribenzoic acid and sodium arsenite. The sulfhydryl reagents and arsenite mask the thiols by forming mercaptides and a DTT-arsenite complex, respectively. The colorimetric and the fluorometric methods can determine Bunte salts in a range of 5×10-9 to 1×10-7 mol and 2×10-8 to 1.5×10-7 mol, respectively.
