Abstract
Comparison of biological and immunological methods for determination of α1-antitrypsin showed that the amount of α1-antitrypsin determined in the presence of trypsin were not the same. From the result of the comparison of effect of heat on the amount of α1-antitrypsin found by biological and immunological methods, it is suggested that α1-antitrypsin and the α1-antitrypsin-trypsin complex are indistinguishable immunologically. The investigation of the trypsin inhibitory activity of α1-antitrypsin and molecular weight determination showed that α1-antitrypsin and trypsin formed a 1 : 1 stoichiometric complex. The results of immunoelectrophoresis and sodium dodecyl sulfate electrophoresis indicated that the α1-antitrypsin-trypsin complex was broken down with incubation and the breakdown products also had immunogenicity.
