Abstract
Kallikreins A and B isolated from hog pancreas showed vasodilator activities of 1350 and 1450 KU/mg (weight) respectively, and same esterolytic activity of 111 units/mg toward BzArgOEt. The sugar content of kallikrein B was roughly twice as much as that of kallikrein A in neutral hexose and glucosamine. Micro-heterogeneous forms B-2 and B-3 which are comprised in kallikrein B showed a 10 times difference in sialic acid content according to their acidities observed in isoelectric focusing. From these and other results obtained by gel plate isoelectric focusing, including the treatment with neuraminidase, it is suggested that the relative acidities on the electrophoresis of various forms of hog pancreatic kallikrein are depending upon the relative amounts of sialic acid and glucosamine in their molecules.
