Abstract
Conformation of dinucleoside monophosphates containing 8-methyladenosine (m8A) residue, which prefer a syn conformation, was analyzed by proton magnetic resonance studies. The dimers are 2'-5' and 3'-5' isomers of Apm8A, and m8Apm8A. The following results were obtained. The data for (2'-5') Apm8A are consistent with an anti (2')-anti (5'), right-handed stacked conformation. The data for (3'-5') Apm8A are consistent with a syn (3')-syn (5') and a high anti (3')-high anti (5') stacked conformation. The data for (3'-5') m8Apm8A can be explained by a syn (3')-syn (5') conformation with little stacking. The data for (2'-5') m8Apm8A can be explained by an anti (2')-syn (5') stacked conformation. These results are in accordance with the results of ultraviolet and circular dichroism studies.
