Preparation and Antigenic Properties of 5α-Dihydrotestosterone-15α-Bovine Serum Albumin Conjugate

抄録

In order to obtain the specific antiserum for the use of radioimmunoassay of 5α-dihydrotestosterone a new hapten-carrier conjugate was prepared from 15α-hydroxy-5α-dihydrotestosterone 15-hemisuccinate by coupling with bovine serum albumin employing the mixed anhydride technique. The specificity of anti-5α-dihydrotestosterone antiserum elicited in the rabbit by immunization with this antigen was tested by cross-reaction studies with the closely related steroids. The results indicated that specific antiserum which is capable of differentiating 5α-dihydrotestosterone from testosterone and 5α-androstane-3β, 17β-diol to a certain extent would be produced by antigen whose steroidal moiety is linked to an immunogenic protein through the position remote from the inherent functional groups on the steroid nucleus.