Abstract
The mycelia of Cochliobolus miyabeanus were treated with hot aqueous phenol and the lipopolysaccharide like substance (LPS-LS) was isolated from aqueous layer. By gel filtration, the LPS-LS was separated to 2 fractions (peak I, peak II) and further investigation was carried out about the peak II fraction and following results were obtained. 1) The molecular weight was about 42000. 2) It consisted of protein (2.0-2.2%), carbohydrate (83-88%), phosphorus (4.5-4.7%), and lipid (6.0-7.5%). 3) Arabinose, mannose, glucose, galactose glucosamine, fatty acids with C14 : 0, C16 : 0, and C18 : 0 were detected in the carbohydrate and lipid moieties, respectively. 4) It indicated low degree of pyrogenicity and antigenicity to rabbits.
