Fluorescence of Boron Complexes. VII. Fluorometric Determination of Salicylamide

Abstract

Fluorometric determination of salicylamide was studied. Salicylamide fluoresced strongly when excited in a mixed solution of 0.1% boric acid, 0.005 N sulfuric acid, and 5% acetic anhydride in glacial acetic acid. This fluorophore was a salicylamide-boron-acetic acid (1 : 1 : 2) complex. The fluorescene emission maximum occurred at 388 nm, and excitation maximum at 328 nm. The linear range in the calibration curve was 5-1000 ng/ml in the final solution, coefficient of variation for salicylamide (0.28μg/ml) in the measurement ten times was 0.6%. This method was about five times more sensitive than the fluorometric method reported by Barr and Riegelman. The proposed method was successfully applied to the determination of salicylamide in urine samples, in which minimum concentration was ca. 30 ppb.