Studies on Transfer Ribonucleic Acids and Related Compounds. XXV. Synthesis of E. coli tRNA^Met_f 5'-Terminal Tetranucleotide C-G-C-Gp and Its Sequence Analog U-G-C-Gp

Abstract

A tetranucleotide C-G-C-Gp (cytidylyl-(3'-5')-guanylyl-(3'-5')-cytidylyl-(3'-5')-guanosine 3'-phosphate) was synthesized by condensation of (Bz) bzC (Bz)-ibG (Bz) p (5) with C (Bz)-ibG (Bz) pNHC₆H₄S (O) CH₃ (6) using 2, 4, 6-triisopropylbenzenesulfonyl chloride. This sequence corresponds to the E. coli tRNA^Met_f 5'-terminus. A base substituted analog U-G-C-Gp was also synthesized by a similar procedure to that used for C-G-C-Gp. These tetranucleotides were characterized by enzymic hydrolysis.