Abstract
A simple and specific method for the determination of moroxydine in serum was established by the use of ion-exchange chromatography and a modified Voges-Proskauer colorimetric reaction. Moroxydine over the range of 5 to 70 μg was determined by the measurement of absorbance at the absorption maximum (520 nm) of the colored product formed in the reaction of moroxydine with diacetyl-3, 5-dihydroxybenzoic acid reagent in alkaline solution. An anion-exchange column chromatography (carboxylate resin) was effectively used to remove the interferences present in the serum. The rabbit serum containing moroxydine was applied directly to the column, without pretreatment, and the column was eluted first with 0.01M NaOH, followed by 0.2M NaOH. The recovery of moroxydine added to serum was between 99 and 102% and the coefficient of variation was between 1.49 and 2.27%.
