Abstract
A radioimmunoassay method has been developed for the determination of theophylline in body fluids. The anti-theophylline antibodies were obtained from the rabbits which were immunized with theophylline-bovine serum albumin conjugate. Standard curve for theophylline determination was linear on logit-logarithm graph in the concentration range of 3 to 50μg/(ml of blood plasma). The plasma samples were collected from theophylline-receiving patients, and analyzed by the method. The values correlated well to those determined by the high-performance liquid chromatography (correlation coefficient 0.981, regression line y=0.96x-0.06). This method was accurate, sensitive, simple and suitable for routine assay of large numbers of samples.
