An Ultramicro Enzymatic Method for the Fluorimetric Determination of Uric Acid in Serum

Abstract

A highly sensitive fluorimetric method is presented for the determination of uric acid in serum, based on the uricase-catalized oxidation of the acid to hydrogen peroxide, which is measured by a fluorimetric technique using the peroxidase-p-hydroxyphenylacetic acid system. The method is rapidly and readily performed with only 2μl of serum without deproteinization.