Abstract
20β-Hydroxysteroid dehydrogenase from Streptomyces hydrogenans is partially inactivated in sodium phosphate buffer within a short time. Inactivation of the enzyme was accelerated at acidic pH, and the enzyme was also inactivated by incubation with rose bengal. The inactivation due to acidic pH and rose bengal was prevented by glycerol ; 20% glycerol completely restored the activity lost at pH 5.5. The mechanism of stabilization of the enzyme by glycerol is not yet clear.
