Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Polynucleotides. LIX. Synthesis and Properties of a Deoxyribohexanucleotide corresponding to the EcoRI Recognition Sequence
EIKO OHTSUKARYOICHI FUKUMOTOMORIO IKEHARA
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1980 Volume 28 Issue 1 Pages 80-84

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Abstract
A deoxyribohexanucleotide corresponding to a restriction endonuclease (Eco RI) recognition sequence, d-G-A-A-T-T-C, was synthesized by stepwise condensation of mononucleotides using dicyclohexylcarbodiimide (DCC) or 2, 4, 6-triisopropylbenzenesulfonyl chloride (TPS) as the condensing reagent. Protected oligonucleotides were separated by ion-exchange chromatography or gel filtration and the deblocked hexanucleotide was purified by DEAE-cellulose column chromatography in the presence of 7 M urea. The product was characterized by enzymatic hydrolysis and its temperature-absorption profile was measured at high ionic strength.
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© The Pharmaceutical Society of Japan
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