抄録
Antigens of 3-O-methylcatecholamines (metanephrine, normetanephrine, 3-methoxy-tyramine) were prepared, keeping the methoxyl and hydroxyl groups of the benzene ring and the side chain intact. Rabbits were immunized with the metanephrine antigen and sufficient antiserum to develop a specific radioimmunoassay of metanephrine was obtained. The detection limit of metanephrine in the radioimmunoassay was 0.03 pmol. The radioimmunoassay was useful for the measurement of urinary metanephrine and of rat liver catechol-O-methyltransferase activity.
