Abstract
The interactions of Sepharose-bound bovine neurophysin I and II with oxytocin and arginine-vasopressin were studied. The amounts of hormones bound to immobilized neurophysin II were found to depend strongly on temperature, being larger at 4° than at 36°. It was found that oxytocin and vasopressin were eluted in different fractions from a column of Sepharose-neurophysin I by shallow pH gradients in either direction from pH 5.8, whereas the hormones emerged in the same fractions from a Sepharose-neurophysin II column.
