Abstract
A quantitative analysis procedure for tetrahydrocannabinolic acid, cannabichromenic acid, cannabidiolic acid and cannabigerolic acid monomethyl ether was established by gas chromatography and by a combined gas chromatography-preparative thin-layer chromatography method using cholestane as an internal standard. The variations of cannabinoid contents with leaf-age, season and sex were investigated in three kinds of "physiological varieties" of Cannabis sativa L., the Mixican, the Minamioshihara No. 1 and the CBDA strain.