Phosphorimetric Determination of α-[(tert-Butylamino)methyl]-3, 4-dihydroxybenzyl Alcohol 3, 4-Di (p-toluate)

Abstract

A phosphorimetric method for the determination of α-[(tert-butylamino)methyl]-3, 4-dihydroxybenzyl alcohol 3, 4-di (p-toluate) (I) was established. The method is based on the native phosphorescence of I in an alkaline extract after separation on a thin-layer chromatographic plate, and can be applied satisfactorily to the determination of I in the concentration range of 0.02-0.5μg per spot.