Studies on the Phosphorimetric Determination of Amines with Halonitro Compounds. III. Phosphorimetric Determination of 4-Homosulfanilamide with 2-Chloro-5-nitropyridine

Abstract

4-Homosulfanilamide was found to react with 2-chloro-5-nitropyridine to form 5-nitro-2-(p-sulfamoylbenzylamino)pyridine, which was strongly phosphorescent in ethanol containing 0.1% (v/v) dimethyl sulfoxide. This observation was used as the basis for a procedure to determine micro amounts of 4-homosulfanilamide, and the method was also applied to the determination of 4-homosulfanilamide added to the rat plasma. A linear relationship between the phosphorescence intensity and the amine concentration was observed in the range of 4×10^-7-2×10^-5M.