Abstract
Ansamitocins and related compounds were isolated from the culture broth of Nocardia sp. 1) The total amount of the antibiotics, after extraction with ethyl acetate from the culture broth, was determined by high-performance liquid chromatography on a μ Porasil column with a solvent mixture of methylene chloride-methanol (20 : 1). 2) Each component in the mixture was quantitatively analyzed on a μ Bondapak column with 69% aqueous methanol. 3) Preparative separation of up to 5 g per cartridge column was carried out in good yield (90%) using a PrepLC/system 500 (Waters) and a column packing of Kieselgel 60 (E. Merck). The purities of the ASM obtained were above 95%.
