Protein Binding of Sulfonylureas. II. Interaction of Some p-Toluenesulfonyl-n-alkylureas with Bovine Serum Albumin

Abstract

To evaluate the substituent effect, the binding of p-toluenesulfonyl-n-alkylureas to bovine serum albumin was studied by equilibrium dialysis and fluorescence titration. The binding constants obtained by fluorescence titration were nearly equal to the geometric means of the primary and secondary binding constants obtained by equilibrium dialysis. The binding constants of p-toluenesulfonyl-n-butylurea were about ten times greater than those of the other compounds tested.