抄録
A sensitive phosphorimetric method is described for the assay of urinary free and total 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG), a major metabolite of norepinephrine. Free MHPG, after separation from urine by Amberlite CG-50 column chromatography, is oxidized with periodate to vanillin, which is then measured phosphorimetrically in a mixture of ether and ethanolic potassium hydroxide. Total MHPG is measured after β-glucuronidase and arylsulfatase mediated hydrolysis of conjugated MHPG. The assay requires less than 2 ml of urine to provide test, blank and standard samples, and offers lower limits of determination of 5 and 30 ng/ml for free and total MHPG, respectively.
