Application of High-performance Liquid Chromatography to the Isolation of Ginsenoside-Rb₁, -Rb₂, -Rc, -Rd, -Re, and -Rg₁ from Ginseng Saponins

Abstract

The major components of ginseng saponins, ginsenoside-Rb₁, -Rb₂, -Rc, -Rd, -Re, and -Rg₁, were isolated from the crude saponin fraction by high-performance liquid chromatography (HPLC), using preparative HPLC on silica gel followed by semi-preparative HPLC on a column packing of Carbohydrate Analysis. This method was rapid and convenient. In addition, ginsenosides could be rapidly analyzed by HPLC on a Carbohydrate Analysis column, which gave excellent resolution.