Studies on Transfer Ribonucleic Acids and Related Compounds. XXXIV. Stepwise Diester or Partial Triester Synthesis of Penta-to Octanucleotides Corresponding to Residues 41-46, 47-54, 61-65 and 66-71 of tRNAMetf of E. coli

EIKO OHTSUKA; TETSUO MIYAKE; EIKO NAKAGAWA; MORIO IKEHARA; ALEXANDER F. MARKHAM

doi:10.1248/cpb.28.2450

Studies on Transfer Ribonucleic Acids and Related Compounds. XXXIV. Stepwise Diester or Partial Triester Synthesis of Penta-to Octanucleotides Corresponding to Residues 41-46, 47-54, 61-65 and 66-71 of tRNA^Met_f of E. coli

大塚栄子, 三宅哲雄, 中川栄子, 池原森男 /, ALEXANDER F. MARKHAM

著者情報

キーワード: gel filtration

ジャーナルフリー

1980 年 28 巻 8 号 p. 2450-2459

DOI https://doi.org/10.1248/cpb.28.2450

詳細

抄録

A hexanucleotide and an octanucleotide corresponding to tRNA^Met_f bases 41-46 and 47-54 were synthesized by stepwise addition of mononucleotides. The octanucleotide is the largest oligoribonucleotide synthesized by this method. A pentanucleotide (bases 61-65) and a hexanucleotide (bases 66-71) were synthesized via partially triesterified intermediates. The deblocked products were purified by ion-exchange chromatography and characterized by enzymatic hydrolysis. These oligonucleotides were used as substrates in joining reactions with RNA ligase.

責任著者(Corresponding author)

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