Abstract
The locations of O-acetyl groups in the acidic polysaccharides AC and BC isolated from the fruit bodies of Tremella fuciformis BERK were determined by employing an improved procedure. The results indicated that the O-acetyl groups in AC and BC are located at positions 4, 6, and both 4 and 6 of a part of the mannose residues, and at positions 2, 4, and both 2 and 4 of a part of the glucuronic acid residues. AC and BC differ significantly in that the amount of the O-acetyl groups linked to the mannose residues in BC was higher than that in AC. Our improved procedure should be widely applicable for determining the locations of O-acyl groups on acidic polysaccharides.
