Author's Organization：Department of Biochemistry, School of Pharmaceutical Sciences, Showa University Department of Biochemistry, School of Pharmaceutical Sciences, Showa University
α-Fetoprotein (AFP) produced by rat AH-66 hepatoma cells was purified and specific antibody was obtained from the serum of an immunized horse. AFP messenger ribonucleic acid (mRNA) (approximately 2700 nucleotides) was isolated by affinity chromatogrophy with oligo (dT)-cellulose of RNA extracted from AFP-synthesizing polysomes of the hepatoma cells.
