Abstract
Biosynthetic incorporation of 2H from [2-13C, 2-2H3]-acetate into 2-hexyl-5-propylresorcinol was investigated in Pseudomonas. The 13C nuclear magnetic resonance spectrum of labelled 2-hexyl-5-propylresorcinol showed 13C-2H signals of expected methylene and methyl carbons in the side chains, but no 2H was found on the aromatic carbons. The main species of methyl groups labelled with 2H were 13C-2H3 and 13C-2H21H. The incorporation experiments unambiguously demonstrate that 2-hexyl-5-propylresorcinol is biosynthesized from two polyketide chains.
