Abstract
Improved chemical synthesis of bovine pancreatic ribonuclease (RNase) A was achieved by applying a new deprotecting procedure with trifluoromethanesulfonic acidthioanisole in combination with a modified air oxidation procedure with glutathione for the disulfide formation. After purifications by affinity chromatography followed by ion-exchange chromatography, a protein with the full enzymatic activity was obtained and subsequently crystallized from 95% ethanol according to Kunitz. A totally synthetic enzyme with full RNase A activity was thus obtained in a crystalline form for the first time.
