Synthesis of the Eicosapeptide corresponding to the N-Terminal Amino Acid Sequence of the Major Component of Human Lymphoblastoid Interferon

Abstract

An eicosapeptide, H-Ser-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Asn-Arg-Arg-Ala-Leu-Ile-Leu-Leu-Ala-Gln-OH, corresponding to the N-terminal amino acid sequence of the major component of human lymphoblastoid interferon was synthesized by a conventional method. The final deprotected peptide was purified by gel filtration on Sephadex G-25 and then by partition chromatography on Sephadex G-25. The eicosapeptide at a dose of 100 μg/ml inhibited the incorporation of ³H-thymidine into DNA of PHA-induced lymphocytes by about 15%.