Abstract
Highly purified hog pancreatic kallikreins A and B from the autolysed hog pancreas were digested with pronase and subjected to hydrazinolysis to release asparagine-linked sugar chains. The oligosaccharides released were separated into fractions based on the acidities due to the contents of neuraminic acid residues. Out of these oligosaccharides, fractions having no neuraminic acid obtained from both A and B were further separated according to the molecular size by the 2 times repeated gel filtrations and their carbohydrate contents were analysed. Thus varied primary structures of carbohydrate chains-namely, different number of neuraminic acid residues being bound, straight and branched oligosaccharide chains, and the high mannose containing and the complex carbohydrates containing structures-of these asparagine-linked oligosaccharides being consisted parts of hog pancreatic kallikreins were observed.
