Abstract
In order to develop specific and sensitive immunoassays, new carboxylated derivatives of androstenedione, 11β-hydroxyandrostenedione, adrenosterone, progesterone, 17α-hydroxyprogesterone, corticosterone, 11-deoxycorticosterone and 21-deoxycortisol were synthesized. The preparation of the 4-carboxymethylthio and 4-carboxyethylthio derivatives of the Δ4-3-ketosteroids was carried out by base-catalyzed ring opening of the 4, 5-epoxides with mercaptoacetic acid and mercaptopropionic acid, respectively. The N-succinimidyl esters of these carboxylated steroids were also prepared.
