Abstract
Acyl trypsins containing the 1-dimethylaminonaphthalene-5-sulfonyl (DNS) fluorophore were prepared in a very specific manner by employing substrates of a new type. The properties of isolated acyl trypsins were spectrofluorometrically analyzed. The deacylation rate of the acyl trypsin, which no longer retains a cationic site-specific group on the acyl group, was enhanced by the addition of alkylammonium ions. This kinetic behavior was shown to be reflected in the fluorescence spectra. These results are discussed on the basis of conformational change of the trypsin active site.
