Human Chorionic Gonadotropin. VII. Preparation and Immunocharacteristics of Carboxyl-terminal Peptides of the β-Subunit of Human Chorionic Gonadotropin

Abstract

Various carboxyl-terminal peptides of hCG-β were prepared from the corresponding blocked peptides by TFA treatment followed by hydrogenation. The prepared peptides were tested for inhibitory action on the binding between ¹²⁵I-hCG and the antiserum against the carboxyl-terminal portion of hCG-β. The results suggest that an antigenic site of this antiserum may exist around the amino-terminal portion of the carboxylterminal hexadecapeptide.