Synthesis of the Nonatriacontapeptide corresponding to the Entire Amino Acid Sequence of Ostrich Adrenocorticotropic Hormone

Abstract

The nonatriaoontapeptide corresponding to the entire amino acid sequence of ostrich adrenocorticotropic hormone (ACTH) was synthesized by successive condensations of four peptide fragments ; Z (OMe)-(15-19)-OH, Z (OMe)-(11-14)-OH, Boc-(5-10)-OH and Z-(1-4)-NHNH₂, with H-(20-39)-OBzl, a synthetic intermediate of ostrich-type corticotropin-like intermediate lobe peptide, followed by deprotection with 1M trifluoromethanesulfonic acid-thioanisole in TFA. The synthetic peptide exhibited an in vivo steroidogenic potency of 0.8 relative to synthetic human ACTH.