抄録
An octanucleotide containing a recognition site for Eco RI and its arabinosyladenine (araA) analog, dGGAATTCC and dGGaraAATTCC, were synthesized by the phosphotriester approach with phosphoro-p-anisidate as the protecting group for 3'-phosphodiesters. araA was converted to 5'-dimethoxytrityl-N, 2'-O-benzoyl 3'-p-chlorophenyl phosphate and condensed with N-benzoyldeoxyadenosine 3'-p-chlorophenyl phosphoro-p-anisidate to yield the protected araAdAp. Other deoxynucleotide blocks (dAAp, dGGp) were prepared similarly and condensed with a 5'-deblocked dTTCC block having the 3'-O-benzoyl group after removal of the p-anisidate group with isoamyl nitrite.
