Abstract
The mechanisms of protein separation by high-performance liquid chromatography (HPLC) using a Shodex OH pak B-804 column were examined, and an attempt was made to relate the separation behavior of some proteins to their molecular weight, isoelectric point and/or molecular size. HPLC elution was carried out with several kinds of buffer solutions at different pH values to cover the range of protein isoelectric points, and the effect of the addition of salts on the elution time was tested. Elution was not necessarily in order of molecular weight or molecular size, and also varied according to whether or not protein-protein, protein-solute and/or protein-column packing interactions occurred in the buffer solutions used.
