CONVENIENT PURIFICATION OF HUMAN SPLEEN FIBRINOLYTIC PROTEINASE (SFP) AND HUMAN LEUCOCYTE ELASTASE BY AFFINITY CHROMATOGRAPHY

Abstract

A peptide inhibitor, Suc-L-Tyr-D-Leu-D-Val-pNA, for human spleen fibrinolytic proteinase (SFP) and for human leucocyte elastase was coupled with AH Sepharose 4B using water soluble carbodiimide. A 116-fold purification of the crude extract of SFP and a 59-fold purification of the partially purified leucocyte elastase were achieved by (Suc-L-Tyr-D-Leu-D-Val-pNA)-AH Sepharose 4B affinity chromatography.