Characterization of Extracellular Mannoheteroglycans from Absidia cylindrospora

抄録

The extracellular polysaccharide fraction of Absidia cylindrospora (ACE), which has been used as an immunogen in immunochemical studies of Mucorales polysaccharide, showed marked heterogeneity of its mannoheteroglycans. ACE was classified into mannantype and fucomannan-type fraotions of sequential separation using affinity chromatography on concanavalin-A (con-A) Sepharose, ion exchange chromatography on diethylaminoethyl Sephadex, zone electrophoresis and gel filtration on Sephadex G-100. Serological reactivity to anti-ACE appeared in the con-A bound and high-mannose content fraction. By methylation analysis and proton magnetic resonanoe studies of each polysaccharide, it was confirmed that the fucomannan-type fraction mainly consisted of α (1→6) linked mannopyranosyl residues and fucopyranosyl non-reducing ends, whereas the mannan-type fraction consisted of (1→2) linked mannopyranosyl residues and mannopyranosyl non-reducing ends. These results were similar to those for mannoheteroglycans from A. cylindrospora mycelium. These results indicated that ACE of the immunogen contained serologically active mannan and fucomannan.