Abstract
Five N-ethyl-N-(2-hydroxy-3-sulfopropyl) aniline derivatives have been synthesized and assessed as water-soluble hydrogen donors for the photometric determination of hydrogen peroxide in the presence of peroxidase. These compounds, sodium salts of N-ethyl-N-(2-hydroxy-3-sulfopropyl) aniline, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-anisidine, 3, 5-dimethyl-N-ethyl-N-(2-hydroxy-3-sulfopropyl) aniline and 3, 5-dimethoxy-N-ethyl-N-(2-hydroxy-3-sulfopropyl)-aniline, gave high absorbances at visible wavelengths in media in the weakly alkaline to fairly acidic pH range. Further, the urea adduct of hydrogen peroxide could be conveniently used as a standard material.
