Abstract
The presence of two main components of porcine pancreatic prokallikrein (prokallikreins A and B) was observed on anion-exchange chromatography. One of them, prokallikrein A, was purified by water extraction, followed by a combination of ammonium sulfate fractionation, ion-exchange chromatographies, preparative disc gel electrophoresis and gel filtration in the presence of various protease inhibitors. Prokallikrein A reacted immunologically with the antibody against kallikrein A obtained from autolyzed porcine pancreas. It migrated slightly more slowly than kallikreins A and B on immunoelectrophoresis. The molecular weight of prokallikrein A was estimated to be 3.6-3.9×104 by high performance liquid chromatography. On the other hand, those of kallikreins A and B were estimated to be 3.3-3.4×104 by the same method. Thus, it was speculated that at least large fragment would not be liberated from the prokallikrein molecule during the activation by protease (s).
