The Active Site of Carboxypeptidase C_U. I. Evidence for Serine in the Active Sites of Carboxypeptidases C_Ua and C_Ub

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Carboxypeptidases C_Ua and C_Ub were both inactivated with incorporation of 1 mol ³²P-labeled diisopropyl fluorophosphate per mol enzyme. The amino acid residue that reacted with this reagent was a serine residue in the active site of each enzyme. The amino acid sequence around this reactive serine residue was determined to be Glu-Gly-Asp-Ser-Gly-Gly-Glu-Leu for both enzymes by sequence analysis of three radioactive peptides isolated from partial acid hydrolysates of the ³²P-labeled enzymes.