Abstract
The binding of 3', 4'-dideoxykanamycin B with a lysosomal enzyme, N-acetyl-β-D-glucosaminidase, obtained from rat kidney was examined by affinity chromatography of the enzyme on 3', 4'-dideoxykanamycin B-conjugated Sepharose 4B. N-Acetyl-β-D-glucosaminidase could be adsorbed on the affinity column. Furthermore, the enzyme activity was significantly increased by the addition of 3', 4'-dideoxykanamycin B. These findings indicate that 3', 4'-dideoxykanamycin B does interact with N-acetyl-β-D-glucosaminidase.
