Abstract
A high-performance liquid chromatographic (HPLC) method for the determination of sultopride in serum and saliva was developed. The limit of detection of sultopride using 0.5ml sample volume was 0.04μg/ml in serum and saliva. The recoveries of sultopride added to serum and saliva (2μg/ml) were 99.6±3% and 99.8±3% (mean±S. D., n=5), respectively. The coefficients of variation of within-run assays for 3 levels of sultopride in serum were less than 4%. There was good agreement between the serum sultopride concentration determined by radioimmunoassay and that determined by HPLC (y=0.961x+0.008, r=0.995).
