抄録
In order to obtain highly purified monoiodohistidine (MIH) in high yield, the optimum iodinating conditions for histidine were examined by HPLC. The isolation and purificaton of MIH from the reaction mixture were attempted by liquid chromatography (LC). The yields of iodohistidines [MIH and diiodohistidine (DIH)] were found to be dependent on the amount and type of the iodinating reagents ; that is, the molar ratio of histidine to iodinating reagent (I2) should be exactly 1 : 1 for MIH and 1 : 2 for DIH for the maximum yield. Purification of MIH was successfully carried out by LC on Dowex 50 (0.05 M 2, 6-lutidine), SEP-PAK C18 cartridge (Waters Associates), and Sephadex G-10 (0.1N AcOH). The overall yield of MIH·HCl obtained by the present method was 57%, which is the highest so far reported.
