Abstract
A sensitive spectrophotometric method for the determination of creatinine was established by using o-hydroxyhydroquinonephthalein (Qn. Ph.)-palladium (II) [Pd (II)] complex in the presence of polyvinyl alcohol (PVA) and sodium dodecyl sulfate (SDS) in weakly acidic media. This method is based on the decrease in absorbance of Qn. Ph. -Pd (II) complex at 615 nm, and could be used in the concentration range of 0.3-4 μg/10 ml of creatinine, where the Sandell sensitivity was estimated to be 0.00053 μg/cm2. Jaffe chromogens scarcely affected the determination of creatinine. Recovery of creatinine added to human urine was examined.
