Studies on Transfer Ribonucleic Acids and Related Compounds. XLIII. Synthesis of Oligoribonucleotides by using 5'-Selective Phosphorylation of 2'-O-Tetrahydrofuranyl Nucleosides

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2'-O-Tetrahydrofuranyl nucleosides have been synthesized from 2, 3-dihydrofuran via 3', 5'-bis-tert-butyldimethylsilylnucleosides. These nucleosides were used as intermediates for oligonucleotide syntheses by the phosphotriester method. Trimers C-C-A and C-C-U were synthesized by the stepwise addition of monomers. U-U-U-U-U-U, A-U-G-A-U-G and A-U-G-A-U-G-A-U-G were obtained by the block condensation of trimers in which the 5'-hydroxyl group had been selectively phosphorylated.