Studies on Transfer Ribonucleic Acids and Related Compounds. XLIV. A Large-Scale Synthesis of the Anticodon Heptanucleotide of Formyl-methionine Transfer Ribonucleic Acid by Using 2'-O-Tetrahydrofuranylnucleosides

Abstract

2'-O-Tetrahydrofuranyl nucleosides were prepared by an improved procedure via 3', 5'-tetraisopropyldisiloxanylnucleosides. A large scale synthesis of the anticodon heptanucleotide C-U-C-A-U-A-A of E. coli formylmethionine tRNA was performed by the phosphotriester method involving condensation of oligonucleotides having 2'-O-tetrahydrofuranyl protecting groups.