Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
A Simultaneous Determination of Norephedrine, Pseudoephedrine, Ephedrine and Methylephedrine in Ephedrae Herba and Oriental Pharmaceutical Preparations by Ion-Pair High-Performance Liquid Chromatography
KAZUHIKO SAGARATOSHIYUKI OSHIMATETSUO MISAKI
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Keywords: crude drugs
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1983 Volume 31 Issue 7 Pages 2359-2365

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Abstract

A new, simple and rapid analytical method using ion-pair high-performance liquid chromatography was developed for simultaneous determination of norephedrine, pseudoephedrine, ephedrine and methylephedrine in Ephedrae Herba and oriental pharmaceutical preparations. A reversed-phase system consisting of an ODS chemically bonded silica gel column and a mixture of water, acetonitrile, sodium dodecyl sulfate and phosphoric acid (65 : 35 : 0.5 : 0.1) as the mobile phase was used. The four components extracted with the mobile phase could be completely separated within 15 min. The detection limits for the four components were 20 ng at a signal-to-noise ratio of 3 : 1.

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© The Pharmaceutical Society of Japan
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